Journal: Non-coding RNA Research

Article Title: Characterization and bioinformatics analysis of ptc-miR396g-5p in response to drought stress of Paeonia ostii

doi: 10.1016/j.ncrna.2022.06.002

Figure Lengend Snippet: Cleavage site of target gene of ‘Feng Dan’ ptc-miR396g-5p from degradome sequencing. The peaks of the signatures at the validated cleavage sites of the corresponding miRNAs were shown in red (dots).

Article Snippet: A miRcute Plus miRNA qPCR Detection Kit (SYBR Green) (TIANGEN, including universal reverse primer) was used for qRT-PCR with a tailing method.

Techniques: Sequencing

Journal: Non-coding RNA Research

Article Title: Characterization and bioinformatics analysis of ptc-miR396g-5p in response to drought stress of Paeonia ostii

doi: 10.1016/j.ncrna.2022.06.002

Figure Lengend Snippet: Expression analysis of ptc-miR396g-5p and target genes. A: Expression analysis of ptc-miR396g-5p (blue) and its target gene (psu.T.00022975, yellow) under different drought stress using quantitative real-time PCR. CK, control, with 28.44% soil moisture content; MD, moderate drought, with 13.27% soil moisture content; SD, severe drought, with 10.93% soil moisture content; and RE, rehydration with 29.82% soil moisture content. B: miRNA expression dynamics detected by RNA sequencing data (yellow) and qRT-PCR (blue). C: Target gene sequencing (yellow) and qRT-PCR (blue).

Article Snippet: A miRcute Plus miRNA qPCR Detection Kit (SYBR Green) (TIANGEN, including universal reverse primer) was used for qRT-PCR with a tailing method.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, RNA Sequencing, Quantitative RT-PCR, Sequencing